Anti-Human EPCAM Recombinant Antibody (Adecatumumab)
CAT#: TAB-106
Recombinant monoclonal antibody to EpCAM. which is used to target tumor cells. It binds to the epithelial cell adhesion molecule (EpCAM - CD326). Adecatumumab has been used in clinical studies of treatment in prostate and breast cancers.
Published Data
Tested Data
Gene Expression
FC
Figure 1 Example of FACS binding analysis for ING-1 binding to transfected CHO cells.
FACS histograms are shown with eight CHO cell transfectants.
Münz, M., Murr, A., Kvesic, M., Rau, D., Mangold, S., Pflanz, S., ... & Rüttinger, D. (2010). Side-by-side analysis of five clinically tested anti-EpCAM monoclonal antibodies. Cancer cell international, 10(1), 44.
FuncS
Figure 2 CDC activity of five clinically tested anti-EpCAM monoclonal antibodies.
Human serum (10%) was added to cultures of KATO III gastric carcinoma cells and CDC after 45 min monitored by TDA released from lysed cells chelated with Europium and quantified by the fluorescence of the Europium-TDA chelates formed.
Münz, M., Murr, A., Kvesic, M., Rau, D., Mangold, S., Pflanz, S., ... & Rüttinger, D. (2010). Side-by-side analysis of five clinically tested anti-EpCAM monoclonal antibodies. Cancer cell international, 10(1), 44.
Inhib
Figure 3 The effect of adecatumumab on proliferation of breast cancer cell line and MCF-7 and normal breast epithelia line MCF10A.
The respective cell lines were seeded at 104 cells and cell numbers determined daily for three days. A human IgG1 isotype mAb and a blank served as controls.
Münz, M., Murr, A., Kvesic, M., Rau, D., Mangold, S., Pflanz, S., ... & Rüttinger, D. (2010). Side-by-side analysis of five clinically tested anti-EpCAM monoclonal antibodies. Cancer cell international, 10(1), 44.
IHC
Figure 4 EpCAM expression by immunohistochemistry on primary/metastatic USPC versus normal endometrial tissue
A representative primary USPC showing strong (3+) EpCAM expression by immunohistochemistry.
El-Sahwi, K., Bellone, S., Cocco, E., Casagrande, F., Bellone, M., Abu-Khalaf, M., ... & Silasi, D. A. (2010). Overexpression of EpCAM in uterine serous papillary carcinoma: implications for EpCAM-specific immunotherapy with human monoclonal antibody adecatumumab (MT201). Molecular cancer therapeutics, 9(1), 57-66.
Cyt
Figure 5 Dose titration cytotoxicity experiments using MT201 against USPC ARK-5.
ADCC was found to plateau at a dose of 5 to 10 μg/mL in multiple experiments.
El-Sahwi, K., Bellone, S., Cocco, E., Casagrande, F., Bellone, M., Abu-Khalaf, M., ... & Silasi, D. A. (2010). Overexpression of EpCAM in uterine serous papillary carcinoma: implications for EpCAM-specific immunotherapy with human monoclonal antibody adecatumumab (MT201). Molecular cancer therapeutics, 9(1), 57-66.
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ELISA
Figure 1 Anti-Human EPCAM Antibody (TAB-106) in ELISA
ELISA analysis of TAB-106 was performed with Human EpCAM Protein, His Tag.
The secondary antibody: HRP-Anti-Human IgG (H+L)
WB
Figure 2 Anti-Human EPCAM Antibody (TAB-106) in WB
Western blot analysis of TAB-106 was performed with Human EpCAM Protein, His Tag.
TAB-106 incubation concentration: 2ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane 1: Reducing antigen (1μg)
DB
Figure 3 Anti-Human EPCAM Antibody (TAB-106) in DB
Dot blot analysis of TAB-106 was performed with Human EpCAM Protein, His Tag.
TAB-106 incubation concentration: 2ng/μL.
NC: Negative control PC: Positive control
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Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67463/1592_H11_2_selected.jpg
Normal Tissue
Figure 2 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_B_2_5.jpg
Normal Tissue
Figure 3 Colon
Endocrine cells
Staining: High
Intensity: Strong
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes - Microvilli
Staining: High
Intensity: Strong
Goblet cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/55098/120585_A_8_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (A-431, CACO-2, CAPAN-2, OE19, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000119888-EPCAM
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Human
- Type
- IgG1
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- FC, IHC, FuncS, Inhib, Cyt
- CAS
- 503605-66-1
- Generic Name
- Adecatumumab
- MW
- 147.5 kDa
- Related Disease
- Breast cancers
Product Property
- Purity
- >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
- Storage
- Store it under sterile conditions at -20°C upon receiving. Recommend to pack the protein into smaller quantities for optimal storage.
Applications
- Application Notes
- The EPCAM antibody has been reported in applications of Inhibition, Flow Cytometry, Immunohistochemistry, Functional Assay, Cytoxicity.
Target
- Alternative Names
- Adecatumumab;503605-66-1;MT201;EPCAM;epithelial cell adhesion molecule;antigen identified by monoclonal AUA1 , M4S1, MIC18, TACSTD1, tumor associated calcium signal transducer 1;17 1A;323/A3;CD326;CO 17A;EGP 2;EGP34;EGP40;Ep CAM;ESA;GA733 2;HEA125;KS1/4;K
- Gene ID
- 4072
- UniProt ID
- P16422
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CITATIONS
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-106, RRID: AB_3111813)
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Biosimilar Overview
Please refer to Adecatumumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Adecatumumab.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Adecatumumab"
Afuco™ Anti-EPCAM ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-106)This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to EpCAM. which is used to target tumor cells. It binds to the epithelial cell adhesion molecule (EpCAM - CD326).
DrugMonitor™ Anti-Adecatumumab Antibody (VS-1224-YC24)Adecatumumab is a recombinant human monoclonal antibody of the IgG1 subclass with specificity for the EpCAM antigen. The DrugMonitor™ Anti-Adecatumumab Antibody (VS-1224-YC24) is an anti-drug antibody (ADA) against Adecatumumab. This drug-based antibody is raised in mice immunized with the Adecatumumab. The anti-Adecatumumab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Adecatumumab in samples.
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| TAB-213 | Humanized Anti-EPCAM Recombinant Antibody (clone Tucotuzumab) | WB, Neut, ELISA, IF, IP, FuncS, FC | Humanized (from mouse) IgG1 |
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| TAB-374MZ | Anti-Human EPCAM Recombinant Antibody (h12H8B) | FC, ELISA | Humanized antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L013E | anti-EPCAM immunotoxin MOC31 (IgG)-PE | Cytotoxicity assay, Functional assay | |
| AGTO-L013D | anti-EPCAM immunotoxin MOC31 (IgG)-DT | Cytotoxicity assay, Functional assay | |
| AGTO-L013G | anti-EPCAM immunotoxin MOC31 (IgG)-Gel | Cytotoxicity assay, Functional assay | |
| AGTO-L013R | anti-EPCAM immunotoxin MOC31 (IgG)-RTA | Cytotoxicity assay, Functional assay | |
| AGTO-L013O | anti-EPCAM immunotoxin MOC31 (IgG)-Bouganin | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-356MZ-S(P) | Anti-Human EPCAM Recombinant Antibody scFv Fragment (3-171) | FC, ELISA | Human antibody |
| TAB-357MZ-S(P) | Anti-Human EPCAM Recombinant Antibody scFv Fragment (7-F17) | FC, ELISA | Human antibody |
| TAB-358MZ-S(P) | Anti-Human EPCAM Recombinant Antibody scFv Fragment (12-C15) | FC, ELISA | Human antibody |
| TAB-359MZ-S(P) | Anti-Human EPCAM Recombinant Antibody scFv Fragment (16-G5) | FC, ELISA | Human antibody |
| TAB-360MZ-S(P) | Anti-Human EPCAM Recombinant Antibody scFv Fragment (17-C20) | FC, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1163 | Hi-Affi™ Rabbit Anti-EPCAM Recombinant Antibody (clone DS1163AB) | IHC-P, WB, ICC, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0129CQ-S(P) | Mouse Anti-EPCAM Recombinant Antibody (clone 17-1A); scFv Fragment | ELISA, IHC, FC | Mouse scFv |
| FAMAB-0130CQ-S(P) | Mouse Anti-EPCAM Recombinant Antibody (clone 323/A3); scFv Fragment | ELISA, IHC, FC | Mouse scFv |
| FAMAB-0131CQ-S(P) | Mouse Anti-EPCAM Recombinant Antibody (clone MOC-161); scFv Fragment | ELISA, IHC, FC | Mouse scFv |
| HPAB-2218LY-S(P) | Human Anti-EPCAM Recombinant Antibody; scFv Fragment (HPAB-2218LY-S(P)) | ELISA | Human scFv |
| HPAB-2219LY-S(P) | Human Anti-EPCAM Recombinant Antibody; scFv Fragment (HPAB-2219LY-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0163CQ | Human Anti-EPCAM Recombinant Antibody (clone HuMAB UBS-54) | ELISA, FC, FuncS | Human IgG1 |
| MRO-0537-CN | Rabbit Anti-EPCAM Recombinant Antibody (clone CBACN-213) | WB, IHC | Rabbit IgG |
| MRO-1842-CN | Rabbit Anti-EPCAM Polyclonal Antibody (MRO-1842-CN) | WB, IF, IHC | Rabbit IgG |
| HPAB-2219LY | Human Anti-EPCAM Recombinant Antibody (HPAB-2219LY) | ELISA | Human IgG |
| HPAB-2220LY | Human Anti-EPCAM Recombinant Antibody (HPAB-2220LY) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0538-CN | Mouse Anti-EPCAM Recombinant Antibody (clone 3-E1-E2) | WB, IF, IHC, FC | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-106 | Afuco™ Anti-EPCAM ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-106) | ELISA, IP, FC, FuncS, Neut | ADCC enhanced antibody |
| AFC-TAB-213 | Afuco™ Anti-EPCAM ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-213) | Neut, ELISA, IF, IP, FuncS | ADCC enhanced antibody |
| AFC-TAB-701 | Afuco™ Anti-EpCAM ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-701) | ELISA, IP, FC, FuncS, IF, Neut | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2216LY-F(E) | Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2216LY-F(E)) | ELISA | Human Fab |
| HPAB-2217LY-F(E) | Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2217LY-F(E)) | ELISA | Human Fab |
| HPAB-2218LY-F(E) | Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2218LY-F(E)) | ELISA | Human Fab |
| HPAB-2219LY-F(E) | Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2219LY-F(E)) | ELISA | Human Fab |
| HPAB-2220LY-F(E) | Human Anti-EPCAM Recombinant Antibody; Fab Fragment (HPAB-2220LY-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0031 | APC-H-2Db/Human Ep-CAM (CLVMKAEM) MHC Tetramer | FCM | |
| MHC-CN0032 | PE-H-2Db/Human Ep-CAM (CLVMKAEM) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1517 | AbPlus™ Anti-EPCAM Magnetic Beads (VS-0724-YC1517) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC37 | Mouse Anti-EPCAM Recombinant Antibody (VS-0924-YC37) - Cancer Stem Cell Marker | IHC, WB | Mouse IgG1 |
| VS-0924-YC38 | Rabbit Anti-EPCAM Antibody (VS-0924-YC38) - Cancer Stem Cell Marker | IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY18 | Human Anti-EpCAM (clone 3-171) scFv-Fc Chimera | Cyt, ELISA, FC, WB | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY107 | CytoStream™ Mouse Anti-EPCAM Recombinant Antibody (VS-0225-XY107) | FC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY773 | Anti-EPCAM Immunohistochemistry Kit | IHC | |
| VS-0525-XY2307 | Anti-Human EPCAM Immunohistochemistry Kit | IHC | |
| VS-0525-XY2308 | Anti-Mouse EPCAM Immunohistochemistry Kit | IHC | |
| VS-0525-XY2309 | Anti-Canine EPCAM Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC210 | Recombinant Anti-EPCAM Vesicular Antibody, EV Displayed (VS-0425-YC210) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC214 | Recombinant Anti-EpCAM (Exon 2 x Exon 5) Biparatopic Antibody, Tandem scFv (Edrecolomab x Adecatumumab) | ELISA, FC | Tandem scFv |
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